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Cyclin D1-dependent recruitment of the ER into a complex with P/CAF. MCF-7 cells were transfected with the indicated combinations of pCX-FLAG-P/CAF (2 μg), pcDNA3.1-hER (1 μg), pRcCMV-cyclin D1 (2 μg), plus the empty pRcCMV vector to make up a total of 6 μg DNA. Immunoprecipitations were carried out with the anti-FLAG antibody. Precipitated proteins were analyzed by SDS/PAGE followed by Western blotting of the indicated antibodies.
