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. 1999 May 11;96(10):5394–5399. doi: 10.1073/pnas.96.10.5394

Figure 5.

Figure 5

Fluorescence microscopy and dose-response curves of CHO DUKX-B11 cells expressing EpoR(1–270)–DHFR fragment fusions. (A) CHO DUKX-B11 cells were stably transfected with EpoR(1–270)–F[1,2] and EpoR(1–270)–F[3] and grown in selective medium in the presence of 2 nM Epo. For microscopy, cells were incubated with fMTX as described for Fig. 3A and then treated with 10 nM Epo or 10 μM EMP1 for 30 min at 37°C. (B) Dose-response curves for Epo and EMP1 were based on flow cytometric analysis of CHO DUKX-B11 cells expressing the same fusions. Mean fluorescence intensities were determined for three separate samples at each ligand concentration: between 0.0003 nM and 100 nM for Epo (triangles) or between 0.0003 μM and 100 μM for EMP1 (circles).