Table 1.
Efficacy of rapamycin-induced survival selection in CHO DUKX-B11 cells
| Ratio of FRB/FKBP cells:wild-type cells | Resistant colonies/ 3 × 106 cells |
|---|---|
| 0:100 | 0 |
| 1:100 | 9,610 |
| 1:1,000 | 896 |
| 1:10,000 | 102 |
| 1:100,000 | 12 |
| 1:1,000,000 | 2 |
CHO DUKX-B11 cells stably expressing FRB-F[1,2] and FKBP-F[3] were mixed at various ratios with wild-type CHO DUKX-B11 cells, in a total of 3 × 106 cells, and incubated in selective medium in the presence of 10 nM rapamycin. The number of colonies was established after 5 days.