Fig. 1.
Identification of a ZFP TF for the regulation of the human CHK2 gene. (A) A schematic representation of the CHK2 promoter indicating the positions of restriction sites and probe used in the DNase I hypersensitive site mapping and the experimentally determined major start site of transcription (Trxn). (B) DNase I hypersensitivity map of the CHK2 promoter. The XbaI and MscI digests serve as location markers, and the numbers indicated on the left refer to the positions of the size markers run alongside. (C) Electrophoretic mobility-shift assay of ZFP-5475 used to determine the apparent in vitro Kd for this DNA-binding protein. Maltose-binding-protein-purified ZFP protein was titrated by using a 3-fold dilution series as indicated.