Fig. 1.

Time course of huPrP23–144 fibrillization monitored by ThT fluorescence. The protein was incubated in the absence (○) or presence (•) of preformed fibrillar seeds (1% wt/wt). (a) Protein incubated at a concentration of 80 μM. (b) Protein incubated at a concentration of 400 μM. Triangles (▴)in b represent the fibrillization kinetics of huPrP23–144 incubated in the presence of fibrillar seeds of synthetic PrP106–126 peptide (5% wt/wt). All samples were in 50 mM potassium phosphate buffer, pH 6.5, at a temperature of 25°C.