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The omitted 2Fo – Fc electron density map of PGI from regions of the proposed substrate-binding site. The map was calculated by omitting Lys-139, Glu-285, His-306, and Lys-420 from the x-ray model. Simulated-annealing refinement was performed with a 3-Å spherical shell of fixed atoms surrounding the omitted regions and was contoured at the 1.0-σ level. Residues His-306′ and Ser-307′ belong to the symmetry-related subunit in a dimer.
