Fig. 5.

Export of HD-82T prevents nuclear accumulation but not cell death. (A) HD-82X construct is identical to HD-82T with the exception of two copies of the export signal (EX) at the N terminus. (B) Absence of HD-82X in the nucleus of either STR or CV-1 does not prevent cell death. Time after transfection is indicated. Images are viewed with a ×32 objective. (C and D) Comparison of the subcellular localization of HD-82T (C) and HD-82X (D)atthe indicated times posttransfection in STR. Images were taken at ×100. (E) Single-cell analysis of STR expressing HD-82X as it progresses toward cell death. Note neurite retraction (Left) and progressive nuclear entry (Right).