Skip to main content
. 2003 Sep 30;100(21):12183–12188. doi: 10.1073/pnas.1635158100

Fig. 2.

Fig. 2.

Loss of CAF-1 leads to slow S-phase progression. (A) CAF-1 knockdown cells pulsed for 10 min (48 h after transfection) or 24 h (36–60 h after transfection) with 40 μM BrdUrd and stained with antibodies specific for BrdUrd and p150. Control cells display high levels of BrdUrd incorporation, whereas CAF-1-depleted cells do not incorporate BrdUrd efficiently (arrows). (B) Two-dimensional flow cytometry analysis monitoring BrdUrd incorporation and DNA content. Cells were pulsed with BrdUrd for 10 min, fixed, and stained with FITC-conjugated anti-BrdUrd antibody and propidium iodide to stain the DNA. BrdUrd intensity is plotted on a log scale on the y axis, and DNA content is plotted on the x axis. G1, G2, and S-phase populations are gated in each sample, and the numbers in the upper right indicate the percentage of BrdUrd-positive cells in each sample.