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. 2003 Oct 2;100(21):12195–12200. doi: 10.1073/pnas.2134310100

Fig. 2.

Fig. 2.

Malformations in Sp8 mutants. (A–H) Alizarin red and alcian blue limb skeletal analysis of E18 fetuses. (A–D) Forelimb skeletons. (A) S, scapula; H, humerus; R, radius; U, ulna. Arrow in B indicates absence of digit 1. (C and D) The humerus forms normally; however, the ulna is distally truncated. The radius is dysplastic (C) or absent (D) and the autopod is absent. (E–H) Caudal portion of fetuses. (E) F, femur. (F–H) Structures distal to the femur are absent. (G) Tail vertebral fusions (arrow). (H) In the Sp8–/– fetus, sacral and caudal vertebrae are absent and the lumbar vertebrae are disorganized and abnormal. (I–M and O) Sp8 null embryos at the ages indicated. (N) Wild type. (I–L) Arrows indicate open anterior neuropore/exencephaly. Arrowheads indicate open posterior neuropore/spina bifida. (I and J) Whole mount in situ with Fgf8 riboprobe arrows indicate ring of cells around open anterior neuropore. (Inset) Frontal view. (M–O) Whole mount in situ with Bf1 riboprobe emphasizing progression of telencephalon tissue through open anterior neuropore.