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. 2003 Oct 6;100(21):12213–12218. doi: 10.1073/pnas.2131679100

Fig. 3.

Fig. 3.

Changes in GABAR function are associated with decreased α1 mRNA expression. (a) aRNA expression profiles from single DGNs acutely isolated from nonhandled (Left) and HMS-30/360 (Right) rats. Slot blot demonstrates hybridization intensities of radiolabeled aRNA probe to a slot blot containing GABAR subunit cDNAs α1–6 (a1–a6), β1–3 (b1–b3), γ1–3 (b4–b6), δ, ε, π, and θ (c1–c4), glial fibrillary acidic protein (c5), neurofilament-L (c6), β-actin (d1), and pBluescript (d2). (b) Mean relative expression (± SEM) of GABAR subunit mRNA as a percentage of total GABAR mRNAs in DGNs isolated from HMS-30/360 vs. nonhandled rats. (c) Mean relative expression of each α-subunit mRNA as a percentage of total α subunit mRNA expression in DGN from HMS-30/360 vs. nonhandled rats (*, P < 0.05; **, P < 0.01, one-way ANOVA with post hoc t tests with Bonferroni correction for multiple comparisons).