Figure 5.

Denaturing PAGE analysis of transcription products in the two transcriptionally incompetent particle types. The position of the short oligonucleotides between five and seven bases in length is indicated with a bracket. Arrowheads (lane 3) indicate the positions of several higher molecular weight RNA species produced in the 2A11/E9 DLP/Fab complex. The band at the bottom of the gel in each lane consists of residual unincorporated radiolabeled GTP not completely removed from the reaction mixture during spin column centrifugation. The intensity of this band is not precisely the same in each lane because of slight differences in the efficiency with which the spin column removed unincorporated radionucleotides after transcription.