Fig. 1.

Amino acid sequence and the expression profile of ERRL1/PGC-1β.(A) LXXLL motifs are shown in bold. Glutamic acid (E) repeats and serine/arginine (SR)-rich regions are underlined. A putative RNA-binding motif is boxed. Vertical lines show tentative domain borders. A splice variant of ERRL1/PGC-1β was identified, which lacks 39 aa (from the 156th leucine to the 194th lysine). The 260th leucine is a proline in reported PGC-1β (17). (B) 3T3-L1 cells were induced to differentiate into adipocytes by treatment with dexamethasone, 1-methyl-3-isobutylxanthine, and insulin (day 0). RNAs were isolated on the days indicated, and Northern blot analysis was performed. The membrane was sequentially hybridized with the probes indicated. (C) Expression of PGC-1β, ERR1, MCAD, and a control GAPDH mRNA in 3T3-L1 and 10T1/2 cells. -, preadipocytes; +, mature adipocytes.