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Cap-binding activity of YB-1. Lysates of CEF expressing the indicated proteins and CEF transfected with RCAS only were analyzed in a m7GTP pull-down assay. Bound FLAG-YB-1 and 4E proteins were detected by Western blotting. A Western blot (WB) analysis using 40 μg of total protein was carried out to confirm the expression of nonbound proteins. eIF, eukaryotic initiation factor.
