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. 2003 Oct 6;100(21):12384–12389. doi: 10.1073/pnas.2135336100

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Copyright © 2003, The National Academy of Sciences

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Fig. 7. — YB-1 inhibits cap-dependent and cap-independent translation. CEF, expressing proteins encoded by RCAS vectors as indicated, were transiently transfected with pHCV-IRES containing the bicistronic reporter CAT/luc. Luciferase activity reflects internal ribosome entry site-dependent translation (); CAT activity represents cap-dependent translation (▪). Experiments were carried out three times; standard deviations are shown as error bars. The translational activity of cells transfected with the empty vector was designated 1.0.

Inline graphic — YB-1 inhibits cap-dependent and cap-independent translation. CEF, expressing proteins encoded by RCAS vectors as indicated, were transiently transfected with pHCV-IRES containing the bicistronic reporter CAT/luc. Luciferase activity reflects internal ribosome entry site-dependent translation (); CAT activity represents cap-dependent translation (▪). Experiments were carried out three times; standard deviations are shown as error bars. The translational activity of cells transfected with the empty vector was designated 1.0.