Table 1. Peptide-binding registers, IC₅₀ values, and T cell recognition of gluten epitopes in the context of HLA-DQ2.5 and HLA-DQ2.2.

	Peptide-binding register, P1—P9									Binding/IC50, μM		T cell stimulation
Gluten epitope	1	2	3	4	5	6	7	8	9	DQ2.5	DQ2.2	DQ2.5	DQ2.2
Glia-α2	P	Q	P	E	L	P	Y	P	Q	15	130	+	—
Glia-α9	P	F	P	Q	P	E	L	P	Y	8	>500	+	—
Glia-α20	F	R	P	E	Q	P	Y	P	Q	30	>500	+	—
Glu-5	E	X	P	E	Q	P	Q	Q	F	100	>500	+	—
Glia-γ2	P	Y	P	E	Q	P	E	Q	P	65	350	+	—
Glia-γ1	P	Q	Q	S	F	P	E	Q	E	14	35	+	+
Glia-γ30	I	I	Q	P	E	Q	P	A	Q	10	50	+	+
Glt-17	P	F	S	E	Q	E	Q	P	V	25	12	+	+
MHCIα*	I	E	Q	E	G	P	E	Y	W	1.6	1.6	ND	ND

The peptides contain glutamic acid residues at positions that are deamidated by tTG. X = I or L. ND, not done.

^*The MHCIα(46—63) reference peptide