Fig. 3.
The Maurer's clefts are intermediates in PfHRPII transport and are targeted by its histidine-rich sequences. Single optical sections of trophozoite-infected cells expressing SSGFPHis154-327 (A-C) or PfHRPIIGFP (D-F) are shown. Cells were fixed, permeabilized, subjected to indirect immunofluorescence assays (see Materials and Methods), and probed with antibodies to GFP (green) and a Maurer's cleft resident protein PfSBP (red). Magnification of the boxes in C and F are shown as C′ and F′, respectively. The extent of colocalization is shown in yellow. In C′ and F′, arrows indicate GFP-labeled tubules or vesicles connecting clefts (arrowheads) to each other or the vacuolar parasite (P). Nuclei were stained with Hoechst (blue). (Scale bar, 5 μm.)