Fig. 3.
IKKα activity in murine PKR-/- and PKR+/+ cells after HSV-1 infection. (A) Electrophoretically separated proteins from lysates of PKR-/- or PKR+/+ cells harvested at 7 h after infection with HSV-1(F) were reacted with anti-ICP27- or anti-US11-specific antibodies. (B) Electrophoretically separated proteins from lysates of PKR-/- or PKR+/+ cells harvested at 2, 4, 6, or 8 h after infection with HSV-1(F) were reacted with anti-IκBα antibody. (C) Autoradiographic images of GST-IκBα reacted with IKKα immunoprecipitated from PKR-/- or PKR+/+ cells 6 h after infection with HSV-1(F). The reaction conditions were as described in Materials and Methods.