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. 2003 Sep 30;100(21):12426–12431. doi: 10.1073/pnas.2134042100

Fig. 2.

Fig. 2.

PA-receptor levels on TIR and non-TIR macrophages and TIR macrophage susceptibility to ET. (A and C) RAW 264.7 cells pretreated with LPS-free 20 mM Tris, pH 8.0. (B and D) RAW 264.7 cells pretreated with 1 μg/ml PA plus 5 ng/ml LF for 24 h. After treatment, cells were incubated for 1 h on ice with 100 nM AM-PA (A and B) or with 100 nM AM-PA plus 200-fold molar excess of cold PA (C and D), washed once, and analyzed by flow cytometry. (E) CHO-R1.1 cells were incubated with AM-PA as above. (F) Control and TIR-RAW 264.7 were treated for 24 h with ET (1 μg/ml PA plus 2 μg/ml EF) and assayed for intracellular cAMP levels.