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. 2003 Oct 6;100(21):12510–12515. doi: 10.1073/pnas.2034966100

Fig. 2.

Fig. 2.

Complementation test for hygromycin sensitivity of 3xHA-tagged constructs in S. cerevisiae Δnhx1 strain. The p426-TEF empty vector was introduced into MM476 (WT/p426TEF) and TY001 (Δnhx1/p426TEF). p426-X1 (Δnhx1/AtNHX1) and pHpl constructs (1–13.2) were transformed into TY001 (pHpl-1 to 13.2). Each strain was grown, and serial dilutions were made as described in Materials and Methods. Three microliters of each dilution (starting from 106 cells) were spotted onto YPD medium and incubated 2 days at 30°C, or onto YPD containing 30 μg/ml hygromycin (YPD+Hyg) and incubated 3 days at 30°C.