Figure 1.

Effect of CO on the anoxic induction of OLE1, CYC7, and COX5b. S. cerevisiae strain JM43 was grown aerobically and then inoculated into a fermentor that was preequilibrated with 2.5% CO₂ in O₂-free N₂ (N₂, open symbols) or 80% CO with 2.5% CO₂ in O₂-free N₂ (CO + N₂, filled symbols). At the indicated times, cells were harvested and total RNA was isolated, fractionated, and hybridized as described in Materials and Methods. The level of each transcript was normalized to ACT1 mRNA, and this ratio is expressed relative to the same ratio in steady state anoxic cells. Data are expressed as means ± error ranges (n = 2).