Table 1.
Effect of CO on expression of oxygen-regulated yeast genes after a shift from normoxic to anoxic conditions
| Gene | Protein | Activator* | Repressor* | O2 effect* | Co effect† |
|---|---|---|---|---|---|
| Oxidative phosphorylation | |||||
| COX5a | Cytochrome c oxidase subunit Va | Hap2/3/4/5 | Induced | None | |
| COX5b | Cytochrome c oxidase subunit Vb | Rox1 | Repressed | Partial inhibition | |
| CYC7 | iso-2-cytochrome c | Hap1 | Rox1 | None/repressed | Total inhibition |
| Heme, sterol, and fatty acid synthesis | |||||
| HEM13 | Coproporphyrinogen III oxidase | Hap1 | Rox1 | Repressed | None |
| HMG1 | 3-hydroxy-3-methylglutaryl CoA reductase | Hap1 | Rox1 | Induced | None |
| HMG2 | 3-hydroxy-3-methylglutaryl CoA reductase | Rox1 | Repressed | None | |
| ERG11 | Cytochrome P450 lanosterol 14α-demethylase | Hap1 | Rox1 | Repressed | None |
| CPR1 (NCP1) | NADPH cytochrome P450 reductase | Rox1 | Repressed | None | |
| OLE1 | Δ-9 fatty acid desaturase | Rox1 | Repressed | Total inhibition | |
| Translation factors | |||||
| TIF51a | elF-5a | Hap1 | Induced | None | |
| ANB1 | elF-5b | Rox1 | Repressed | None | |
| Mitochondrial adenine translocases | |||||
| AAC2 | ADP/ATP translocator | Hap2/3/4/5 | Induced | None | |
| AAC3 | ADP/ATP translocator | Rox1 | Repressed | None | |
| Transcriptional regulators | |||||
| ROX1 (REO1) | DNA-binding transcriptional repressor | Hap1 | Induced | None | |
*
Hypoxic genes are repressed by Rox 1 p in air; aerobic genes and some hypoxic genes are activated (induced) by either Hap1 or Hap2/3/4/5p (20).
†
Determined by quantitating the effect of CO on transcript levels every 2 or 4 h over a 24 h period.