Figure 1.
Differences in DnaJ requirements for stimulation of ATP hydrolysis by DnaK and luciferase refolding. (A) Effect of DnaJ on stimulation of ATP hydrolysis. Hydrolysis rates dependent on DnaJ (filled diamonds) were determined by quenched flow experiments and yielded a fitted maximal value of 0.79 s−1. The open diamond indicates the additional effect of σ32 (final concentration 2 μM) at the highest DnaJ concentration. (B) Efficiency of luciferase refolding by the DnaK system dependent on DnaJ. Chemically denatured luciferase was refolded in buffer containing constant concentrations of DnaK, GrpE, and ATP, and various concentrations of DnaJ. The lines between A and B indicate the differences in the DnaJ concentration used in the two experiments.