Figure 1.

The growth curve and the expression of PSA in HER2/Neu stable transfected LNCaP. A HER2/Neu stable transfected LNCaP cell line was established by transfection with HER2/Neu or its parent vector under 400 μg/ml G418 selection. (A and B) Cells were seeded at 2 × 10⁵ on a six-well plate. The cells were counted every 24 h by MTT assay. Each count represents the average of four independent experiments. (C) The blot containing 30 μg of total RNA isolated from cells on each lane was hybridized with a PSA cDNA probe. The 28S RNA shows the equal loading of RNA.