Figure 2.

Induction of AR transactivation by HER2/Neu in the presence of androgen in DU145 cells. One microgram of human AR alone or 2.5 μg of pCMVHER2/Neu and/or 2.5 μg of pSG5ARAs was transfected into DU145 cells. PSA-CAT, which contains the 2.8 kb 5′-promoter region of PSA gene, was used as the AR-target reporter gene. Cells were treated with 1 nM DHT after transfection. Cells were harvested after 24 h for CAT assay, and relative CAT activity was normalized by the β-galactosidase activity and quantitated by image- quant software. Data represent an average of three independent experiments.