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. 1999 May 11;96(10):5458–5463. doi: 10.1073/pnas.96.10.5458

Figure 4.

Figure 4

(A) Inhibition of the HER2/Neu-mediated AR transactivation by AG879 or PD98059. (B) Suppression of AR transactivation by CL100. (A) One microgram of human pSG5AR or 3 μg of pSG5ARAs and/or 3 μg of pCMV-HER2/Neu was transfected into DU145 cells. MMTV-CAT was used as the AR-target reporter gene. After transfection, the cells were treated with or without DHT, 10 μM AG879, or 15 μM PD98059 for another 24 h, and cells were then harvested for CAT assay. (B) One microgram of human pSG5AR or 3 μg of pSG5ARA55 and/or 3 μg of pCMV HER2/Neu plus CL100 was transfected into DU145 cells for 24 h, and the cells were mock treated or treated with 10−11 or 10−9 M DHT for 24 h and then harvested for CAT assay. Values represent the mean ± SD of at least three determinations.