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Promotion of the interaction of AR and ARAs by HER2/Neu. The DU145 cells were transiently cotransfected with 2.5 μg of reporter plasmids, pG5-LUC, 2.5 μg of Gal4DBD fused to ARA70 amino acids 176–401 (Gal4-ARA70), and 2.5 μg of VP16-AR fusion in the presence or the absence of HER2/Neu. DHT was then added at 1 nM for 24 h before cells were harvested for luciferase (LUC) assay.
