Figure 2.

Northern blot analysis of plant RRF homologue. (A) Tissue-specific expression of RRFHCP. Northern blots were performed on total RNA (10 μg each) from cotyledons (C), leaves (L), hypocotyls (H), and roots (R) isolated from 20-day-old spinach plants. L1, 2, and 3 represent different ages of leaves, with L1 as the oldest (approximately 10 days). (B) Expression of RRFHCP is not light-dependent. Lanes: 1, 18 days in the dark (D); 2, 14 days in the dark followed by 4 days in the light (D+L); 3, 18 days in the light (L). Methylene blue staining of total RNA and serial hybridizations with different probes were used as loading controls (not shown). The ³²P-labeled DNA probe corresponds to nucleotides 269–477 of RRFHCP cDNA.