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The E. coli RRF reaction is inhibited by mature RRFHCP. The activity of wild-type RRF (10 μg, ○; 60 μg, ●) and temperature-sensitive RRF (60 μg, ▵) is expressed as a percentage of that which occurs in the absence of the inhibitor. This is plotted against the molar ratio of mature RRFHCP to E. coli RRF. Because of the low activity of the temperature-sensitive RRF, it was not technically possible to use 10 μg of it.
