Table 1.

Strains and plasmids

Name	Characteristics
Strains
LJ2708	LJ3 transformed with pPEN1054sacBneo plasmid
LJ2211	LJ3 transformed with pPEN1054sacBneo and pMR2 plasmids
LJ2846	MC1061 harboring the F′ from XL1-Blue MRF′
LJ2221	LJ14 harboring the F′ from XL1-Blue MRF′
Plasmids
pKK233-2RRFM	pKK233–2 derivative carrying a fragment of the plant frr cDNA as a 0.58-kbp NcoI fragment encoding the mature part of the plant RRF
pET15b-RRFM	pET15b derivative carrying a fragment of the plant frr cDNA as a 0.55-kbp BamHI fragment encoding the mature part of the plant RRF fused to an N-terminal His-tag sequence
pCRII-RRF	pCRII derivative carrying a fragment of the plant frr cDNA as a 0.9-kbp EcoRI fragment (nucleotide residues 1–898 of the plant cDNA)
pPEN1054	pBR322 carries frr (Wt: wild type) as a 0.9-kb EcoRI-SmaI fragment from pRR1 between its EcoRI-ScaI sites; tetracyclin resistance; ColE1 replicon
pPEN1054 sacBneo	pPEN1054 carries the sacB-Neor cassette from pIB279 as a 3.1-kb BamHI fragment inserted into its single BamHI site; Kmr; ColE1 replicon