Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Chromatographic resolution of products of acylation with six aa-AMP–RNA 77 reactions are at pH 7.25 and 0°C with 1–5 mM adenylate. Phe and Tyr reactions are for 0.5 min, all others for 20 min. RNA 29 reactions are similar but terminated at 2 min. The 3′ [32P] nucleotide was released from aa-RNAs by P1 nuclease digestion and aminoacylated and unacylated forms resolved by cellulose polyethyleneimine chromatography.
