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. 1990 Aug 1;172(2):641–643. doi: 10.1084/jem.172.2.641

The B cell antigen CD75 is a cell surface sialytransferase

PMCID: PMC2188328  PMID: 2373995

Abstract

In this work we have isolated a cDNA clone encoding the B cell antigen CD75. The amino acid sequence of CD75 is shown to be identical to that of human alpha 2,6 sialyltransferase, believed to be primarily associated with the Golgi complex. This is the first demonstration of cell surface expression of sialytransferase which, in B cells, may play an important role in intercellular adhesion and antigen presentation events.

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Selected References

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  1. Ansorge W., Sproat B. S., Stegemann J., Schwager C. A non-radioactive automated method for DNA sequence determination. J Biochem Biophys Methods. 1986 Dec;13(6):315–323. doi: 10.1016/0165-022x(86)90038-2. [DOI] [PubMed] [Google Scholar]
  2. Ansorge W., Sproat B., Stegemann J., Schwager C., Zenke M. Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis. Nucleic Acids Res. 1987 Jun 11;15(11):4593–4602. doi: 10.1093/nar/15.11.4593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aruffo A., Seed B. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8573–8577. doi: 10.1073/pnas.84.23.8573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Auffray C., Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. doi: 10.1111/j.1432-1033.1980.tb06030.x. [DOI] [PubMed] [Google Scholar]
  5. Blomhoff H. K., Smeland E. B., Beiske K., Blomhoff R., Ruud E., Bjøro T., Pfeifer-Ohlsson S., Watt R., Funderud S., Godal T. Cyclic AMP-mediated suppression of normal and neoplastic B cell proliferation is associated with regulation of myc and Ha-ras protooncogenes. J Cell Physiol. 1987 Jun;131(3):426–433. doi: 10.1002/jcp.1041310315. [DOI] [PubMed] [Google Scholar]
  6. Edelman G. M. Cell adhesion and the molecular processes of morphogenesis. Annu Rev Biochem. 1985;54:135–169. doi: 10.1146/annurev.bi.54.070185.001031. [DOI] [PubMed] [Google Scholar]
  7. Grundmann U., Nerlich C., Rein T., Zettlmeissl G. Complete cDNA sequence encoding human beta-galactoside alpha-2,6-sialyltransferase. Nucleic Acids Res. 1990 Feb 11;18(3):667–667. doi: 10.1093/nar/18.3.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kozak M. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984 Jan 25;12(2):857–872. doi: 10.1093/nar/12.2.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lance P., Lau K. M., Lau J. T. Isolation and characterization of a partial cDNA for a human sialyltransferase. Biochem Biophys Res Commun. 1989 Oct 16;164(1):225–232. doi: 10.1016/0006-291x(89)91706-3. [DOI] [PubMed] [Google Scholar]
  10. Paulson J. C., Colley K. J. Glycosyltransferases. Structure, localization, and control of cell type-specific glycosylation. J Biol Chem. 1989 Oct 25;264(30):17615–17618. [PubMed] [Google Scholar]
  11. Powell L. D., Whiteheart S. W., Hart G. W. Cell surface sialic acid influences tumor cell recognition in the mixed lymphocyte reaction. J Immunol. 1987 Jul 1;139(1):262–270. [PubMed] [Google Scholar]
  12. Seed B., Aruffo A. Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure. Proc Natl Acad Sci U S A. 1987 May;84(10):3365–3369. doi: 10.1073/pnas.84.10.3365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stamenkovic I., Seed B. Analysis of two cDNA clones encoding the B lymphocyte antigen CD20 (B1, Bp35), a type III integral membrane protein. J Exp Med. 1988 Jun 1;167(6):1975–1980. doi: 10.1084/jem.167.6.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Taira S., Kakiuchi T., Minami M., Nariuchi H., Taiara S. The regulatory role of sialic acids in the response of class II reactive T cell hybridomas to allogeneic B cells. J Immunol. 1986 Oct 15;137(8):2448–2454. [PubMed] [Google Scholar]
  15. Warren L., Buck C. A., Tuszynski G. P. Glycopeptide changes and malignant transformation. A possible role for carbohydrate in malignant behavior. Biochim Biophys Acta. 1978 Sep 18;516(1):97–127. doi: 10.1016/0304-419x(78)90005-7. [DOI] [PubMed] [Google Scholar]
  16. Weinstein J., Lee E. U., McEntee K., Lai P. H., Paulson J. C. Primary structure of beta-galactoside alpha 2,6-sialyltransferase. Conversion of membrane-bound enzyme to soluble forms by cleavage of the NH2-terminal signal anchor. J Biol Chem. 1987 Dec 25;262(36):17735–17743. [PubMed] [Google Scholar]
  17. Yednock T. A., Rosen S. D. Lymphocyte homing. Adv Immunol. 1989;44:313–378. doi: 10.1016/s0065-2776(08)60645-8. [DOI] [PubMed] [Google Scholar]
  18. von Heijne G. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 1986 Jun 11;14(11):4683–4690. doi: 10.1093/nar/14.11.4683. [DOI] [PMC free article] [PubMed] [Google Scholar]

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