Figure 4.

Effects of GRK2, the GRK2 C terminus, and phosducin on PTH receptor-stimulated [³H]inositol phosphate accumulation. COS-1 cells were transiently transfected with the PTH receptor alone (control) or together with kinase-inactive GRK2-K220R, the G protein βγ-subunit binding GRK2 C terminus (GRK2-CT), or the high-affinity G protein βγ-subunit binding protein phosducin. [³H]inositol phosphate accumulation in response to 100 nM PTH for 1 h was measured. Data are mean ± SE (n = 4).