Figure 6.

Crosslinking of GRK2 to the PTH receptor (PTHR). The HA-tagged PTH receptor or the T480 mutant receptor was transfected alone (leftmost lane) or together with GRK2 into COS-1 cells. The cells were incubated for 10 min with or without 100 nM PTH and crosslinking with 2.5 mM dithiobis(succinimidyl propionate) was done as described in Materials and Methods. The receptors were solubilized and immunoprecipitated with anti-HA antibodies, and coprecipitated GRK2 was detected in Western blots.