Abstract
A new Duffy specificity, Fy6, defined by a murine monoclonal antibody of the IgG1 kappa class, is related to susceptibility to malarial invasion. In humans, Fy6 is present on the red cells of all persons except those of the Fy(a-b-) type, a distribution resembling that of Fy3. However proteolytic enzyme treatment of red cells enhances the reactivity of Fy3, whereas Fy6, like Fya and Fyb, is susceptible to degradation by this process. The number of Fy6 sites on human red cells was found to be 12,200 per cell, in close agreement with earlier estimates of the number of Fya sites. Anti-Fy6 reacted in western blots with a membrane glycoprotein of approximately 46,000 Mr, not significantly different from that of a molecule known to bear the Fya determinant. The Fy6 epitope is shown to be present on the red cells of some but not all nonhuman primate species, where it has a distribution not only distinctly different from Fya, Fyb, and Fy3, but in close accordance with susceptibility to penetration by Plasmodium vivax. Thus, the red cells of two species of macaques (Macaca mulatta and M. fascicularis), which are invaded by Plasmodium knowlesi but not by P. vivax are shown to have other Duffy antigens but to be devoid of Fy6. It appears, therefore, that the red cell epitopes used by these closely related species are distinct, and that susceptibility to P. vivax merozoite penetration is dependent on the presence of Fy6.
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Selected References
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- Albrey J. A., Vincent E. E., Hutchinson J., Marsh W. L., Allen F. H., Jr, Gavin J., Sanger R. A new antibody, anti-Fy3, in the Duffy blood group system. Vox Sang. 1971 Jan;20(1):29–35. doi: 10.1111/j.1423-0410.1971.tb01797.x. [DOI] [PubMed] [Google Scholar]
- BIRD G. W. ANTI-T IN PEANUTS. Vox Sang. 1964 Nov-Dec;9:748–749. doi: 10.1111/j.1423-0410.1964.tb04072.x. [DOI] [PubMed] [Google Scholar]
- Behzad O., Lee C. L., Gavin J., Marsh W. L. A new anti-erythrocyte antibody in the Duffy system: anti-Fy4. Vox Sang. 1973 Apr;24(4):337–342. doi: 10.1111/j.1423-0410.1973.tb02651.x. [DOI] [PubMed] [Google Scholar]
- Colledge K. I., Pezzulich M., Marsh W. L. Anti-Fy5, and antibody disclosing a probable association between the Rhesus and Duffy blood group genes. Vox Sang. 1973 Mar;24(3):193–199. doi: 10.1111/j.1423-0410.1973.tb02630.x. [DOI] [PubMed] [Google Scholar]
- Donahue R. P., Bias W. B., Renwick J. H., McKusick V. A. Probable assignment of the Duffy blood group locus to chromosome 1 in man. Proc Natl Acad Sci U S A. 1968 Nov;61(3):949–955. doi: 10.1073/pnas.61.3.949. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hadley T. J., David P. H., McGinniss M. H., Miller L. H. Identification of an erythrocyte component carrying the Duffy blood group Fya antigen. Science. 1984 Feb 10;223(4636):597–599. doi: 10.1126/science.6695171. [DOI] [PubMed] [Google Scholar]
- IKIN E. W., MOURANT A. E., PETTENKOFER H. J., BLUMENTHAL G. Discovery of the expected haemagglutinin, anti-Fyb. Nature. 1951 Dec 22;168(4288):1077–1078. doi: 10.1038/1681077b0. [DOI] [PubMed] [Google Scholar]
- Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Marsh W. L. Present status of the Duffy blood group system. CRC Crit Rev Clin Lab Sci. 1975 Mar;5(4):387–412. doi: 10.3109/10408367509107049. [DOI] [PubMed] [Google Scholar]
- Mason S. J., Miller L. H., Shiroishi T., Dvorak J. A., McGinniss M. H. The Duffy blood group determinants: their role in the susceptibility of human and animal erythrocytes to Plasmodium knowlesi malaria. Br J Haematol. 1977 Jul;36(3):327–335. doi: 10.1111/j.1365-2141.1977.tb00656.x. [DOI] [PubMed] [Google Scholar]
- Masouredis S. P., Sudora E., Mahan L., Victoria E. J. Quantitative immunoferritin microscopy of Fya, Fyb, Jka, U, and Dib antigen site numbers on human red cells. Blood. 1980 Dec;56(6):969–977. [PubMed] [Google Scholar]
- Miller L. H., Mason S. J., Clyde D. F., McGinniss M. H. The resistance factor to Plasmodium vivax in blacks. The Duffy-blood-group genotype, FyFy. N Engl J Med. 1976 Aug 5;295(6):302–304. doi: 10.1056/NEJM197608052950602. [DOI] [PubMed] [Google Scholar]
- Miller L. H., Mason S. J., Dvorak J. A., McGinniss M. H., Rothman I. K. Erythrocyte receptors for (Plasmodium knowlesi) malaria: Duffy blood group determinants. Science. 1975 Aug 15;189(4202):561–563. doi: 10.1126/science.1145213. [DOI] [PubMed] [Google Scholar]
- Miller L. H., McAuliffe F. M., Mason S. J. Erythrocyte receptors for malaria merozoites. Am J Trop Med Hyg. 1977 Nov;26(6 Pt 2):204–208. doi: 10.4269/ajtmh.1977.26.204. [DOI] [PubMed] [Google Scholar]
- Nichols M. E., Rosenfield R. E., Rubinstein P. Two blood group M epitopes disclosed by monoclonal antibodies. Vox Sang. 1985;49(2):138–148. doi: 10.1111/j.1423-0410.1985.tb00782.x. [DOI] [PubMed] [Google Scholar]
- SANGER R., RACE R. R., JACK J. The Duffy blood groups of New York negroes: the phenotype Fy (a-b-). Br J Haematol. 1955 Oct;1(4):370–374. doi: 10.1111/j.1365-2141.1955.tb05523.x. [DOI] [PubMed] [Google Scholar]
- Young M. D., Baerg D. C., Rossan R. N. Parasitological review. Experimental monkey hosts for human plasmodia. Exp Parasitol. 1975 Aug;38(1):136–152. doi: 10.1016/0014-4894(75)90046-6. [DOI] [PubMed] [Google Scholar]
- Young M. D., Porter J. A., Jr, Johnson C. M. Plasmodium vivax transmitted from man to monkey to man. Science. 1966 Aug 26;153(3739):1006–1007. doi: 10.1126/science.153.3739.1006. [DOI] [PubMed] [Google Scholar]