Figure 3.
Bax, but not Bcl-xL, decreases membrane lifetime. (A) Lifetimes of DOPC:DOPE:DOPS (1:1:1) bilayers were measured at 250 mV, in the presence of full-length Bax, full-length Bcl-xL, and reticulocyte control. The final concentration of Bax and Bcl-xL was 150 pM. Standard errors are shown for 10–15 experiments. There was no systematic change in lifetime between membranes painted in the presence of Bax and those to which Bax was freshly added. (B) Membrane lifetime as a function of full-length Bax (F.L. Bax), full-length Bcl-xL (F.L. Bcl-xL), and truncated Bax (ΔC22-Bax).