Figure 4.

Endogenous Bax from bovine thymus decreases membrane lifetime. (A) Purification of Bax from bovine thymocytes. Bovine Bax was purified from thymocyte soluble protein fraction by conventional column chromatography and α Bax 2D2 immunoaffinity chromatography followed by elution by using a peptide containing the antibody epitope. The resultant eluent was analyzed by SDS/PAGE and silver staining (S.S.) or by Western blotting by using α uBax 2D2 monoclonal antibody (W.B.). (B) Effect of bovine Bax on DOPC:DOPE:DOPS (1:1:1) membrane lifetime. The holding potential was 250 mV.