Figure 5.
Bcl-xL does not protect against Bax-induced membrane destabilization. (A) Bax and Bcl-xL do not form stable complexes in the reticulocyte lysate. Bax and Bcl-xL were coexpressed in the reticulocyte, and a sample of the reaction was immunoprecipitated with a monoclonal antibody against Bax. The presence of Bax and Bcl-xL in the reticulocyte lysate (lane 1), immunoprecipitate (lane 2) and in the supernatant (lane 3) was checked by SDS/PAGE and Western blotting, by using monoclonal antibodies against both proteins. (B) Decrease in the lifetime induced by Bax expressed alone or together with Bcl-xL. Bax and Bcl-xL concentrations were 150 pM.