Table 1.
Effect of Bcl-xL on Bax-induced membrane destabilization
| Protein | Concentration, nM | Lifetime, s |
|---|---|---|
| None | NA | 12.8 ± 3.5 |
| Bcl-xL | 0.25 | 11.7 ± 2.9 |
| Bax | 0.25 | 0.41 ± 0.14 |
| *Bcl-xL/Bax | 0.25/0.25 | 0.47 ± 0.13 |
| †Bcl-xL + Bax | 0.25 + 0.25 | 0.25 ± 0.09 |
| †Bcl-xL + Bax | 0.50 + 0.25 | 0.34 ± 0.17 |
| †Bcl-xL + Bax | 1.00 + 0.25 | 0.15 ± 0.04 |
| ‡Bcl-xL + Bax | 2.50 + 0.25 | 0.41 ± 0.10 |
*
Bcl-xL and Bax coexpressed in the reticulocyte lysate.
†
Bcl-xL and Bax expressed separately in the reticulocyte lysate. Bcl-xL was first incubated for 5 min in the chamber followed by Bax addition.
‡
As in † except that Bcl-xL expressed in bacteria.