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Transplantation of ROSA26 testis cells. (A) Testis from ROSA26 transgenic mouse incubated in X-gal showing blue staining of donor cells. (B) Testis from a C57BL/6 × 129/SvF1 recipient mouse demonstrating that cells do not stain. (C) Recipient testis 2 months after transplantation of ROSA26 donor cells. Blue stretches of seminiferous tubules represent colonies formed by donor spermatogonial stem cells that have expanded to produce areas of spermatogenesis. (Bar = 2 mm.)
