Figure 4.

Flow cytometric analysis of anti-β₁-integrin antibody selected cells. (A) Fluorescence of β₁-integrin selected cells treated with FITC-conjugated antibody was compared with fluorescence of similar cells (controls) not treated with FITC-conjugated antibody. The shaded area shows the range of fluorescence of controls. Fluorescence was measured in arbitrary units on a log scale. The average percent of high fluorescing β₁-integrin cells above the control range (shaded area) in three experiments was 51.3 ± 11.9 (mean ± SEM). The value for unselected cells was 3.7 ± 1.2 (not shown). (B) Cells selected as in A were treated with anti-c-kit antibody conjugated to PE and analyzed as above. In three experiments, the average percent of high fluorescing c-kit-expressing cells above controls in the anti-β₁-integrin antibody selected cell population was 8.0 ± 3.5 (mean ± SEM). The value for unselected cells was 7.8 ± 2.1 (not shown).