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CDC53 can be modified by NEDD8 in yeast. Western blot developed with Xpress-tag-specific antibodies of immunoprecipitates with HA-specific antibodies from yeast cells carrying vector (control), plasmids expressing HANEDD8, or HANEDD8 plus Xpress-tagged CDC53 (HXCDC53; Invitrogen). Immunoprecipitation of HANEDD8 results in a coprecipitation of HANEDD8-modified HXCDC53.
