Figure 4.

hCUL-1 and hCUL-2 are modified by NEDD8 in vivo. (A) Human 293 cells transiently transfected either with empty vector (−) or with pcDNA3–^3mycNEDD8 were lysed, and proteins were analyzed by immunoblotting with anti-hCUL-1 antibodies (Left) or anti-hCUL-2 antibodies (Right). (B) As in A, 293 cells transiently transfected either with empty vector (−) or with pcDNA3–^3mycNEDD8 were lysed and proteins were immunoprecipitated with anti-myc (Left), anti-hCUL-2 (Right, two left lanes), or anti-myc antibodies (Right, two right lanes), followed by Western analysis with anti-hCUL-1 (Left) or anti-hCUL-2 antibodies (Right).