Figure 5.

The zinc finger domain of Nup-358 binds to RanGDP, and binding is prevented by zinc chelation. (A) Immobilized Nup-358-ZF protein (see Fig. 1), either untreated or EDTA-treated, was incubated with or without RanGDP. Bound and unbound fractions were visualized by SDS/PAGE and Coomassie blue staining. (B) Immobilized Nup-358-ZF protein, either untreated or EDTA-treated, was incubated with [β-³²P]RanGDP. Bound radioactivity was measured and expressed as a percentage of total Ran-associated radioactivity in the reaction.