Skip to main content
. 1999 May 11;96(10):5522–5527. doi: 10.1073/pnas.96.10.5522

Figure 3.

Figure 3

The LEF-1-binding sequence in the cyclin D1 promoter is required for β-catenin-mediated transactivation. (A) Cells from the 293T line were transfected with the indicated reporter plasmid (0.8 μg), together with 4 μg of ΔN-β-catenin or the control plasmid (pCIneo). The promoter activity is presented as in Fig. 1B. (B) Schematic representation of mutations in the −163 cyclin D1 promoter construct, including an AT to GC change at nucleotides −75 and −74 (−163mtLefCD1LUC) and deletion of the LEF-1 binding site (−163ΔLefCD1LUC) between nucleotides −81 to −73. (C) Neuro 2A cells were transfected with 0.8 μg of −1745CD1Luc, 2 μg of ΔN-β-catenin, or a control plasmid (pCIneo), along with increasing amounts of a LEF-1 expression plasmid. DNA concentrations were kept constant with empty vector DNA. Transfections were carried out in triplicate and the means ± SD are presented.