Figure 3.

Interaction of HSL with ALBP in vivo. Extracts of rat epididymal fat (lane 3) were immunoprecipitated with preimmune antibodies, or extracts of rat epididymal fat (lane 4) or extracts of CHO cells stably overexpressing HSL (lane 5) were immunoprecipitated with anti-HSL antibodies, as described in Materials and Methods. The immunoprecipitated pellets were separated on SDS/15% PAGE, immunoblotted with anti-mouse ALBP antibodies (A) or anti-rat HSL fusion protein antibodies (B), and visualized by using enhanced chemiluminescence. Lane 1 contains purified ALBP (0.8 μg) and lane 2 contains extracts (10 μg) of adipose tissue homogenate before immunoprecipitation.