Figure 6.

Physical and functional analysis of NS5A–Grb2 interaction in an inducible stable NS5A expression HeLa cell system. (A) EGF-induced ERK1/2 phosphorylation in NS5A- or non-NS5A-expressing HeLa cells at different time points after EGF treatment (20 ng/ml, 2 min). Phosphorylation and protein levels of ERK1/2 were measured as described in Fig. 5B. (B) Anti-NS5A immunoblot comparing total lysate with anti-Grb2 or NMS-precipitated protein complexes from HeLa cells stably expressing NS5A with (+) or without (−) EGF stimulation.