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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: J Neuroimmunol. 2007 Sep 27;192(1-2):21–30. doi: 10.1016/j.jneuroim.2007.08.006

Figure 1. Semiquantitative RT-PCR analysis of Taar1 mRNA expression in mouse macrophages and dendritic cells (no DNase).

Figure 1

Apparent mRNA expression of Taar1 in mouse macrophages and dendritic cells during LPS activation, in the absence of DNase treatment of the total RNA. The mRNA expression of the housekeeping gene, GAPDH, is used as a positive control to indicate that similar amounts of cDNA are present in each sample. IL-6 mRNA expression is used to demonstrate cell activation. RT-PCR was performed using total RNA and the results presented as amplified products electrophoresed on ethidium bromide stained agarose gels. DNA sizes in base pairs are shown to the left of the DNA standard. Putative Taar1 expression in the absence of DNase digestion is also shown for liver and brain.