Figure 4.

Comparison of PC containing neuropeptide with neuropeptide immunoreactivity in fractions from the HPLC of the rat placental extract. Fractions 30–65 from the preparative HPLC step in Fig. 3 were submitted to a number of two-site ELISAs. –◊– represents two-site anti-neuropeptide/anti-neuropeptide assay results in each case. All ELISA protocols were the same as described in Materials and methods; (a) proCRF, in (b) NKB, in (c) activin A and (d) hemokinin. –•– represents the results of two-site immunometric assays with the anti-neuropeptide antibody being in (a) anti-proCRF (1–21) IgG, (b) anti-NKB(1–5) IgG, (c) anti-proβA subunit IgG and (d) anti-HK(1–5) IgG in combination with anti-PC IgG in each case.