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. 2007 Jul 24;39(3):189–198. doi: 10.1677/JME-07-0007

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© 2007 Society for Endocrinology

This is an Open Access article distributed under the terms of the Society for Endocrinology's Re-use Licence which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The effect of enzyme treatment on the immunoreactivity of pro-CRF-PC and pro-βA-PC. Peak one represents a pool of fractions 36–37 and peak two, a pool of fractions 41–43 from Fig. 4, which were freeze-dried separately before being reconstituted in assay buffer. Open histogram represents ELISA assay result with no enzyme treatment, black histogram after phospholipase C digestion and grey histogram after endoglycosidase digestion. Values are the means of duplicate determinations in the respective two-site immunometric assay.