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. 2008 Jan;172(1):225–235. doi: 10.2353/ajpath.2008.070065

Figure 2.

Figure 2

ARA70α and ARA70β function as AR co-activators in prostate cell lines. PC3 cells were transiently transfected with AR and 4XARE-Luc or PSA-Luc reporter genes along with increasing amounts of ARA70α or ARA70β or empty expression vector to equalize the total amount of DNA per condition. Cells were treated with 10 nmol/L R1881 (+) or ethanol vehicle (−) for 12 hours and receptor transcriptional activation was assayed as described in Materials and Methods, normalized for transfection efficiency, and expressed as relative luminescence units (RLUs). The average of three independent experiments and SE is shown. A: Transcriptional activation of ARA70α and ARA70β on 4XARE-Luc-reporter gene. B: Transcriptional activation of ARA70β on PSA-Luc reporter gene. C: Antagonist effects of Casodex on ARA70α transcriptional activation. D: Antagonist effects of Casodex on ARA70β transcriptional activation.